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American Society of Microbiology ABMM Sample Questions:

1. A patient with AIDS develops a disseminated fungal infection. Blood cultures grow a yeast that produces pseudohyphae and terminal chlamydospores on cornmeal agar. The patient's CD4+ T-cell count is below 50 cells/µL. The MOST likely causative organism is:

A) Cryptococcus neoformans
B) Candida albicans
C) Pneumocystis jirovecii
D) Aspergillus fumigatus

2. A research team is investigating the mechanisms of antifungal resistance in Candida albicans. They identify a strain that exhibits resistance to azole antifungal agents. Further analysis reveals overexpression of a specific ATP-binding cassette (ABC) transporter protein in the resistant strain. This mechanism of resistance involves:

A) Modification of the fungal cell wall, preventing azole penetration.
B) Alteration of the fungal ergosterol biosynthesis pathway, the target of azoles.
C) Increased degradation of the azole antifungal agent within the fungal cell.
D) Active efflux of the azole antifungal agent out of the fungal cell.

3. A patient with a history of travel to a rural area develops a febrile illness with jaundice and dark urine.
Peripheral blood smear reveals intraerythrocytic parasites with a characteristic Maltese cross morphology.
The MOST likely causative agent is:

A) Trypanosoma brucei
B) Plasmodium falciparum
C) Babesia microti
D) Plasmodium vivax

4. Microscopic examination of a trichrome-stained stool smear reveals amoebic trophozoites approximately 25
µm in diameter, containing ingested red blood cells. The nucleus, when visible, has fine, evenly distributed peripheral chromatin and a small, central karyosome. These features are diagnostic for:

A) Entamoeba histolytica
B) Endolimax nana
C) Iodamoeba bütschlii
D) Entamoeba coli

5. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

A) The number of true positive results divided by the total number of positive test results.
B) The number of true positive results divided by the total number of true positive and false negative results.
C) The number of true negative results divided by the total number of negative test results.
D) The number of true negative results divided by the total number of true negative and false positive results.

Solutions: